DNA binding mediated by the wheat HMGa protein: a novel instance of selectivity against alternating GC sequence
Identifieur interne : 003580 ( Main/Exploration ); précédent : 003579; suivant : 003581DNA binding mediated by the wheat HMGa protein: a novel instance of selectivity against alternating GC sequence
Auteurs : Chua Yii Leng [Singapour] ; Pwee Keng Hock [Singapour] ; R. Manjunatha Kini [Singapour]Source :
- Plant Molecular Biology [ 0167-4412 ] ; 2001-05-01.
English descriptors
- KwdEn :
Abstract
Abstract: The high-mobility-group (HMG) chromosomal protein wheat HMGa was purified to homogeneity and tested for its binding characteristics to double-stranded DNA. Wheat HMGa was able to bind to P268, an A/T-rich fragment derived from the pea plastocyanin gene promoter, producing a small mobility shift in gel retardation assays where the bound complex was sensitive to addition of proteinase K but resistant to heat treatment of the protein, consistent with the identity of wheat HMGa as a putative HMG-I/Y protein. Gel retardation assays and southwestern hybridization analysis revealed that wheat HMGa could selectively interact with the DNA polynucleotides poly(dA).poly(dT), poly(dAdT).poly(dAdT), and poly(dG).poly(dC), but not with poly(dGdC).poly(dGdC). Surface plasmon resonance analysis determined the kinetic and affinity constants of sensor chip-immobilized wheat HMGa for double-stranded DNA 10-mers, revealing a good affinity of the protein for various dinucleotide combinations, except that of alternating GC sequence. Thus contrary to prior reports of a selectivity of wheat HMGa for A/T-rich DNA, the protein appears to be able to interact with sequences containing guanine and cytosine residues as well, except where G/C residues alternate directly in the primary sequence.
Url:
DOI: 10.1023/A:1010696604330
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000074
- to stream Istex, to step Curation: 000074
- to stream Istex, to step Checkpoint: 000F02
- to stream Main, to step Merge: 003618
- to stream Main, to step Curation: 003580
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">DNA binding mediated by the wheat HMGa protein: a novel instance of selectivity against alternating GC sequence</title><author><name sortKey="Yii Leng, Chua" sort="Yii Leng, Chua" uniqKey="Yii Leng C" first="Chua" last="Yii Leng">Chua Yii Leng</name></author><author><name sortKey="Keng Hock, Pwee" sort="Keng Hock, Pwee" uniqKey="Keng Hock P" first="Pwee" last="Keng Hock">Pwee Keng Hock</name></author><author><name sortKey="Kini, R Manjunatha" sort="Kini, R Manjunatha" uniqKey="Kini R" first="R. Manjunatha" last="Kini">R. Manjunatha Kini</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:CEC1471031E22FD0AA4A4F4D4E074363743AE8CF</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1023/A:1010696604330</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-8VRKBZR7-D/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000074</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000074</idno><idno type="wicri:Area/Istex/Curation">000074</idno><idno type="wicri:Area/Istex/Checkpoint">000F02</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F02</idno><idno type="wicri:doubleKey">0167-4412:2001:Yii Leng C:dna:binding:mediated</idno><idno type="wicri:Area/Main/Merge">003618</idno><idno type="wicri:Area/Main/Curation">003580</idno><idno type="wicri:Area/Main/Exploration">003580</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">DNA binding mediated by the wheat HMGa protein: a novel instance of selectivity against alternating GC sequence</title><author><name sortKey="Yii Leng, Chua" sort="Yii Leng, Chua" uniqKey="Yii Leng C" first="Chua" last="Yii Leng">Chua Yii Leng</name><affiliation wicri:level="1"><country xml:lang="fr">Singapour</country><wicri:regionArea>Plant Molecular Biology Laboratory</wicri:regionArea></affiliation></author><author><name sortKey="Keng Hock, Pwee" sort="Keng Hock, Pwee" uniqKey="Keng Hock P" first="Pwee" last="Keng Hock">Pwee Keng Hock</name><affiliation wicri:level="1"><country xml:lang="fr">Singapour</country><wicri:regionArea>Plant Molecular Biology Laboratory</wicri:regionArea></affiliation></author><author><name sortKey="Kini, R Manjunatha" sort="Kini, R Manjunatha" uniqKey="Kini R" first="R. Manjunatha" last="Kini">R. Manjunatha Kini</name><affiliation wicri:level="4"><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Biological Sciences, National University of Singapore, Block S1A, 10 Science Drive 4, 117543, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Molecular Biology</title><title level="j" type="sub">An International Journal on Molecular Biology, Molecular Genetics and Biochemistry</title><title level="j" type="abbrev">Plant Mol Biol</title><idno type="ISSN">0167-4412</idno><idno type="eISSN">1573-5028</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="2001-05-01">2001-05-01</date><biblScope unit="volume">46</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="193">193</biblScope><biblScope unit="page" to="204">204</biblScope></imprint><idno type="ISSN">0167-4412</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0167-4412</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>A/T-rich</term><term>G/C-rich</term><term>high-mobility-group (HMG) proteins</term><term>southwestern hybridization</term><term>surface plasmon resonance analysis</term><term>wheat HMGa</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The high-mobility-group (HMG) chromosomal protein wheat HMGa was purified to homogeneity and tested for its binding characteristics to double-stranded DNA. Wheat HMGa was able to bind to P268, an A/T-rich fragment derived from the pea plastocyanin gene promoter, producing a small mobility shift in gel retardation assays where the bound complex was sensitive to addition of proteinase K but resistant to heat treatment of the protein, consistent with the identity of wheat HMGa as a putative HMG-I/Y protein. Gel retardation assays and southwestern hybridization analysis revealed that wheat HMGa could selectively interact with the DNA polynucleotides poly(dA).poly(dT), poly(dAdT).poly(dAdT), and poly(dG).poly(dC), but not with poly(dGdC).poly(dGdC). Surface plasmon resonance analysis determined the kinetic and affinity constants of sensor chip-immobilized wheat HMGa for double-stranded DNA 10-mers, revealing a good affinity of the protein for various dinucleotide combinations, except that of alternating GC sequence. Thus contrary to prior reports of a selectivity of wheat HMGa for A/T-rich DNA, the protein appears to be able to interact with sequences containing guanine and cytosine residues as well, except where G/C residues alternate directly in the primary sequence.</div></front></TEI><affiliations><list><country><li>Singapour</li></country><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="Singapour"><noRegion><name sortKey="Yii Leng, Chua" sort="Yii Leng, Chua" uniqKey="Yii Leng C" first="Chua" last="Yii Leng">Chua Yii Leng</name></noRegion><name sortKey="Keng Hock, Pwee" sort="Keng Hock, Pwee" uniqKey="Keng Hock P" first="Pwee" last="Keng Hock">Pwee Keng Hock</name><name sortKey="Kini, R Manjunatha" sort="Kini, R Manjunatha" uniqKey="Kini R" first="R. Manjunatha" last="Kini">R. Manjunatha Kini</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003580 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003580 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:CEC1471031E22FD0AA4A4F4D4E074363743AE8CF
|texte= DNA binding mediated by the wheat HMGa protein: a novel instance of selectivity against alternating GC sequence
}}
| This area was generated with Dilib version V0.6.33. |  |